Fluorescent lamps

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using ellman's reagent or bimane derivatives such as monobromobimane . the monobromobimane method is more sensitive. in this procedure, cells are lysed and thiols extracted using a hcl buffer . the thiols are then reduced with dithiothreitol and labelled by monobromobimane. monobromobimane becomes fluorescent

the major limitation of these bimane-based probes and many other reported probes is that these probes are based on irreversible chemical reactions with glutathione, which renders these probes incapable of monitoring the real-time glutathione dynamics. recently, the first reversible reaction based fluorescent...

https://en.wikipedia.org/wiki/Glutathione

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