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collagenase (sigma, typei) in nominally ca + -free tytode solution, followed for ~ min. the softened heart was removed from the column, and the left ventricle was dissected in modified kb (kcl mm, kh po mm, mgso mm, koh mm, glutamate mm, taurine mm, hepes mm, glucose mm, egta mm, and ph with koh). the cells
peak current and i-v curve were recorded (t ). the microperfusion tube was then swithed by another reservoir prefilled with μmol/l bupivacaine and ug/ml epinephrine after the model was successfully made by μmol/l bupivacaine. the peak current and i-v curve were then recorded at the time (t ) when the cells...
https://bmcanesthesiol.biomedcentral.com/articles/10.1186/s12871-015-0049-1