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to locate and identify genes that are involved in cellular response, such as in signaling pathways, or to discover the function of novel genes. pooled screening libraries can vary in scale from as few as constructs, up to hundreds of thousands. in contrast to an arrayed approach, pooled screens do not
normally require extensive and costly automation equipment. instead, with the appropriate experimental design, a pooled approach allows researchers to screen a population of modified cells with just a few culture plates. cloning, trans formation, transfection, packaging, transduction and screening are...
https://www.agilent.com/cs/library/brochures/CRISPR%20Brochure%205991-9158EN.pdf
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