Sound reproducing using optical media

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covered and incubated at °c for hr. . content of the wells was discarded and wells were washed times with μl of washing solution. . μl of substrate a and substrate b each was added to each well. the plate was covered and incubated at °c for min. . μl of the stop solution was added per well. . the optical

density (od value) of each well was read immediately using a microplate reader set to nm. . the duplicate readings for each sample were averaged and the average zero standard optical density subtracted. the corrected average-value was tabulated as average absorbance. a standard curve was generated by...

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